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dna amplification efficiencies  (Thermo Fisher)


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    Thermo Fisher dna amplification efficiencies
    Dna Amplification Efficiencies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+amplification+efficiencies/DNA/pm38176658-97-15-21
    Average 99 stars, based on 1 article reviews
    dna amplification efficiencies - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    DNA Amplification:

    Article Title: Integrating rapid pathogen identification and antimicrobial susceptibility testing through multiplex TaqMan qPCR assay.
    Article Snippet: Timely bacterial identification (ID) and antimicrobial susceptibility testing (AST) are of significance for therapy of bacteria-infected patients.. In the present study, we developed a multiplex TaqMan qPCR assay for rapid and accurate ID and AST of three common hospital acquired pneumonia species, namely Acinetobacter baumannii, Klebsiella pneumoniae and Staphylococcus aureus.. In this assay, DNA extraction and bacterial co-incubation with antibiotics are accomplished based on a common PCR instrument.

    Article Title: Testing device, microfluidic chip and nucleic acid testing method
    Article Snippet: .. No. mRNA Target CFX96 Touch TM Microfluidic Chip 1 AST - Control 20.07 ± 0.02 20.5 ± 0.2 2 AST - 3x Dosage 19.34 ± 0.05 19.9 ± 0.2 3 ALT - Control 27.28 ± 0.10 28.8 ± 0.2 4 ALT - 3x Dosage 26.75 ± 0.07 28.5 ± 0.3 5 β-Actin - Control 22.93 ± 0.11 23.0 ± 0.2 6 β-Actin - 3x Dosage 23.02 ± 0.08 23.1 ± 0.2 The presence of the housekeeping gene, β-actin, allowed the inventors to normalize the qPCR experimental data, to minimize possible variations from the experimental setup such as sample preparation and handling, reverse transcription efficiency between sample-sample/run-run repetitions, and DNA amplification efficiencies (M. T. Dorak, Real-time PCR, Taylor & Francis, 2007; Life Technologies Corporation, Real-time PCR: Understanding Ct). ..

    Lysis:

    Article Title: Integrating rapid pathogen identification and antimicrobial susceptibility testing through multiplex TaqMan qPCR assay.
    Article Snippet: Timely bacterial identification (ID) and antimicrobial susceptibility testing (AST) are of significance for therapy of bacteria-infected patients.. In the present study, we developed a multiplex TaqMan qPCR assay for rapid and accurate ID and AST of three common hospital acquired pneumonia species, namely Acinetobacter baumannii, Klebsiella pneumoniae and Staphylococcus aureus.. In this assay, DNA extraction and bacterial co-incubation with antibiotics are accomplished based on a common PCR instrument.

    DNA Extraction:

    Article Title: Integrating rapid pathogen identification and antimicrobial susceptibility testing through multiplex TaqMan qPCR assay.
    Article Snippet: Timely bacterial identification (ID) and antimicrobial susceptibility testing (AST) are of significance for therapy of bacteria-infected patients.. In the present study, we developed a multiplex TaqMan qPCR assay for rapid and accurate ID and AST of three common hospital acquired pneumonia species, namely Acinetobacter baumannii, Klebsiella pneumoniae and Staphylococcus aureus.. In this assay, DNA extraction and bacterial co-incubation with antibiotics are accomplished based on a common PCR instrument.

    AST Assay:

    Article Title: Testing device, microfluidic chip and nucleic acid testing method
    Article Snippet: .. No. mRNA Target CFX96 Touch TM Microfluidic Chip 1 AST - Control 20.07 ± 0.02 20.5 ± 0.2 2 AST - 3x Dosage 19.34 ± 0.05 19.9 ± 0.2 3 ALT - Control 27.28 ± 0.10 28.8 ± 0.2 4 ALT - 3x Dosage 26.75 ± 0.07 28.5 ± 0.3 5 β-Actin - Control 22.93 ± 0.11 23.0 ± 0.2 6 β-Actin - 3x Dosage 23.02 ± 0.08 23.1 ± 0.2 The presence of the housekeeping gene, β-actin, allowed the inventors to normalize the qPCR experimental data, to minimize possible variations from the experimental setup such as sample preparation and handling, reverse transcription efficiency between sample-sample/run-run repetitions, and DNA amplification efficiencies (M. T. Dorak, Real-time PCR, Taylor & Francis, 2007; Life Technologies Corporation, Real-time PCR: Understanding Ct). ..

    Control:

    Article Title: Testing device, microfluidic chip and nucleic acid testing method
    Article Snippet: .. No. mRNA Target CFX96 Touch TM Microfluidic Chip 1 AST - Control 20.07 ± 0.02 20.5 ± 0.2 2 AST - 3x Dosage 19.34 ± 0.05 19.9 ± 0.2 3 ALT - Control 27.28 ± 0.10 28.8 ± 0.2 4 ALT - 3x Dosage 26.75 ± 0.07 28.5 ± 0.3 5 β-Actin - Control 22.93 ± 0.11 23.0 ± 0.2 6 β-Actin - 3x Dosage 23.02 ± 0.08 23.1 ± 0.2 The presence of the housekeeping gene, β-actin, allowed the inventors to normalize the qPCR experimental data, to minimize possible variations from the experimental setup such as sample preparation and handling, reverse transcription efficiency between sample-sample/run-run repetitions, and DNA amplification efficiencies (M. T. Dorak, Real-time PCR, Taylor & Francis, 2007; Life Technologies Corporation, Real-time PCR: Understanding Ct). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Testing device, microfluidic chip and nucleic acid testing method
    Article Snippet: .. No. mRNA Target CFX96 Touch TM Microfluidic Chip 1 AST - Control 20.07 ± 0.02 20.5 ± 0.2 2 AST - 3x Dosage 19.34 ± 0.05 19.9 ± 0.2 3 ALT - Control 27.28 ± 0.10 28.8 ± 0.2 4 ALT - 3x Dosage 26.75 ± 0.07 28.5 ± 0.3 5 β-Actin - Control 22.93 ± 0.11 23.0 ± 0.2 6 β-Actin - 3x Dosage 23.02 ± 0.08 23.1 ± 0.2 The presence of the housekeeping gene, β-actin, allowed the inventors to normalize the qPCR experimental data, to minimize possible variations from the experimental setup such as sample preparation and handling, reverse transcription efficiency between sample-sample/run-run repetitions, and DNA amplification efficiencies (M. T. Dorak, Real-time PCR, Taylor & Francis, 2007; Life Technologies Corporation, Real-time PCR: Understanding Ct). ..

    Sample Prep:

    Article Title: Testing device, microfluidic chip and nucleic acid testing method
    Article Snippet: .. No. mRNA Target CFX96 Touch TM Microfluidic Chip 1 AST - Control 20.07 ± 0.02 20.5 ± 0.2 2 AST - 3x Dosage 19.34 ± 0.05 19.9 ± 0.2 3 ALT - Control 27.28 ± 0.10 28.8 ± 0.2 4 ALT - 3x Dosage 26.75 ± 0.07 28.5 ± 0.3 5 β-Actin - Control 22.93 ± 0.11 23.0 ± 0.2 6 β-Actin - 3x Dosage 23.02 ± 0.08 23.1 ± 0.2 The presence of the housekeeping gene, β-actin, allowed the inventors to normalize the qPCR experimental data, to minimize possible variations from the experimental setup such as sample preparation and handling, reverse transcription efficiency between sample-sample/run-run repetitions, and DNA amplification efficiencies (M. T. Dorak, Real-time PCR, Taylor & Francis, 2007; Life Technologies Corporation, Real-time PCR: Understanding Ct). ..

    Reverse Transcription:

    Article Title: Testing device, microfluidic chip and nucleic acid testing method
    Article Snippet: .. No. mRNA Target CFX96 Touch TM Microfluidic Chip 1 AST - Control 20.07 ± 0.02 20.5 ± 0.2 2 AST - 3x Dosage 19.34 ± 0.05 19.9 ± 0.2 3 ALT - Control 27.28 ± 0.10 28.8 ± 0.2 4 ALT - 3x Dosage 26.75 ± 0.07 28.5 ± 0.3 5 β-Actin - Control 22.93 ± 0.11 23.0 ± 0.2 6 β-Actin - 3x Dosage 23.02 ± 0.08 23.1 ± 0.2 The presence of the housekeeping gene, β-actin, allowed the inventors to normalize the qPCR experimental data, to minimize possible variations from the experimental setup such as sample preparation and handling, reverse transcription efficiency between sample-sample/run-run repetitions, and DNA amplification efficiencies (M. T. Dorak, Real-time PCR, Taylor & Francis, 2007; Life Technologies Corporation, Real-time PCR: Understanding Ct). ..



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    Comparison of AOM and Qiagen extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using <t>HSV-positive</t> CSF. Neat HSV1-PC+ was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines. The QDM extracted HSV-1 <t>DNA</t> from the serial dilutions was amplified and detected in AOM tubes (dashed lines). HSV-negative CSF was extracted by the AOM method and represented by the light solid line. AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.
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    Image Search Results


    Comparison of AOM and Qiagen extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using HSV-positive CSF. Neat HSV1-PC+ was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines. The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes (dashed lines). HSV-negative CSF was extracted by the AOM method and represented by the light solid line. AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.

    Journal:

    Article Title: A Single-Tube Nucleic Acid Extraction, Amplification, and Detection Method Using Aluminum Oxide

    doi: 10.2353/jmoldx.2006.040398

    Figure Lengend Snippet: Comparison of AOM and Qiagen extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using HSV-positive CSF. Neat HSV1-PC+ was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines. The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes (dashed lines). HSV-negative CSF was extracted by the AOM method and represented by the light solid line. AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.

    Article Snippet: HSV DNA Amplification Efficiency in AOM Tubes To determine whether the assembled AOM tube adversely impacted PCR, a quantified HSV-1 DNA standard (ABI) was used to examine real-time PCR amplification efficiency in AOM tubes compared to standard PCR tubes.

    Techniques: Comparison, Extraction, Amplification, Real-time Polymerase Chain Reaction

    Real-time PCR amplification of HSV-1 DNA in AOM tubes. One thousand copies of quantified HSV-1 DNA (ABI) were used to compare crossing thresholds between standard PCR and AOM tubes. PCR control: PCR tube control reaction. AOM control: AOM tube reaction with 5 mmol/L phosphate in master mix. AOM filtered: AOM tube reaction with DNA filtered onto the membrane with 5 mmol/L phosphate in master mix. AOM filtered (low phos): AOM tube reaction with DNA filtered onto the membrane with 0.75 mmol/L phosphate in master mix. No DNA control: AOM tube with no DNA with 5 mmol/L phosphate in master mix. All reactions run in triplicate.

    Journal:

    Article Title: A Single-Tube Nucleic Acid Extraction, Amplification, and Detection Method Using Aluminum Oxide

    doi: 10.2353/jmoldx.2006.040398

    Figure Lengend Snippet: Real-time PCR amplification of HSV-1 DNA in AOM tubes. One thousand copies of quantified HSV-1 DNA (ABI) were used to compare crossing thresholds between standard PCR and AOM tubes. PCR control: PCR tube control reaction. AOM control: AOM tube reaction with 5 mmol/L phosphate in master mix. AOM filtered: AOM tube reaction with DNA filtered onto the membrane with 5 mmol/L phosphate in master mix. AOM filtered (low phos): AOM tube reaction with DNA filtered onto the membrane with 0.75 mmol/L phosphate in master mix. No DNA control: AOM tube with no DNA with 5 mmol/L phosphate in master mix. All reactions run in triplicate.

    Article Snippet: HSV DNA Amplification Efficiency in AOM Tubes To determine whether the assembled AOM tube adversely impacted PCR, a quantified HSV-1 DNA standard (ABI) was used to examine real-time PCR amplification efficiency in AOM tubes compared to standard PCR tubes.

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Control, Membrane

    Dynamic range and extraction efficiency of AOM tubes. Quantified HSV-positive CSF (HSV1-PC+, 100 to 100,000 copies of HSV-1 per ml of CSF) dilutions were processed using the AOM method (solid line) and compared to quantified HSV-1 DNA (ABI) dilutions filtered into AOM tubes (dashed line, 100 to 100,000 copies of HSV-1 per ml). Error bars represent a single SD. All reactions run in triplicate.

    Journal:

    Article Title: A Single-Tube Nucleic Acid Extraction, Amplification, and Detection Method Using Aluminum Oxide

    doi: 10.2353/jmoldx.2006.040398

    Figure Lengend Snippet: Dynamic range and extraction efficiency of AOM tubes. Quantified HSV-positive CSF (HSV1-PC+, 100 to 100,000 copies of HSV-1 per ml of CSF) dilutions were processed using the AOM method (solid line) and compared to quantified HSV-1 DNA (ABI) dilutions filtered into AOM tubes (dashed line, 100 to 100,000 copies of HSV-1 per ml). Error bars represent a single SD. All reactions run in triplicate.

    Article Snippet: HSV DNA Amplification Efficiency in AOM Tubes To determine whether the assembled AOM tube adversely impacted PCR, a quantified HSV-1 DNA standard (ABI) was used to examine real-time PCR amplification efficiency in AOM tubes compared to standard PCR tubes.

    Techniques: Extraction